Journal: Aging (Albany NY)

Article Title: Anillin is required for tumor growth and regulated by miR-15a/miR-16-1 in HBV-related hepatocellular carcinoma

doi: 10.18632/aging.101510

Figure Lengend Snippet: MiR-15a and miR-16-1 mediate the downregulation of ANLN expression in HCC cells. ( A ) Western blotting analysis of ANLN protein expression in HepG2 and HepG2.215 cells. ( B ) QPCR analysis of ANLN mRNA expression in HepG2 and HepG2.215 cells. ( C ) Endogenous ANLN protein turnover in HepG2 and HepG2.215 cells over the course of 10 h following the addition of 200 μg·mL -1 cycloheximide. GAPDH indicates total protein loading for each sample. The relative fold changes of each sample compared with time 0 are shown below. ( D ) Potential microRNAs targeting full-length ANLN mRNA are shown using the TargetScan tool ( www.targetscan.org ). ( E ) The endogenous expression levels of miR-15a, miR-16-1, miR-195 and miR-424 in HepG2 and HepG2.215 cells were determined using qPCR. U6 was used as an internal control. ( F ) Western blotting analysis of ANLN expression in HepG2.215 cells treated with miR-15a, miR-16-1, miR-195 and negative control mimics, respectively. The relative fold changes compared with control are shown below. ( G ) Western blotting analysis of ANLN expression in HepG2 cells treated with miR-15a, miR-16-1, miR-195 and negative control microRNA inhibitors, respectively. The asterisk indicates a nonspecific band. The relative fold changes compared with the control are shown below. (H) Schematic diagram of the reporter constructs containing the predicted miR-15a and miR-16-1 binding sites in the 3’ UTR of ANLN. ( I ) Treatment of miR-15a and miR-16-1 mimics significantly attenuated the luciferase activity of the ANLN 3’ UTR compared with the control in QGY-7703 cells. *P < 0.05; ***P < 0.001.

Article Snippet: The miR-15a, miR-16-1, miR-195 anti-sense inhibitors and a non-specific miRNA inhibitor control were synthesized by GenePharma.

Techniques: Expressing, Western Blot, Control, Negative Control, Construct, Binding Assay, Luciferase, Activity Assay

Journal: Oncotarget

Article Title: Cancer-associated fibroblasts promote cancer cell growth through a miR-7-RASSF2-PAR-4 axis in the tumor microenvironment

doi: 10.18632/oncotarget.13609

Figure Lengend Snippet: A. Heat map of differentially expressed microRNA in CAFs compared with NFs. B. The expression levels of 6 miRNAs (miR-7, miR-365, miR-103a, miR-335, miR-196a and miR10a) in 8 pairs of NFs and CAFs. A log2-fold change more than 2 was regarded as significant up-regulation (dotted lines). C. A volcano plot showing the relationship between the P values and the magnitude of the differences in the expression values of the samples in different groups. D. A real-time PCR analysis was performed to validate the miR-7 expression in 8 paired samples. (*** p <0.001.)

Article Snippet: The miRNA expressions were analyzed using SYBR Green methods and a miRcute miRNA qPCR kit according to the manufacturer's protocol (TIANGEN, Shanghai, China).

Techniques: Expressing, Real-time Polymerase Chain Reaction